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Nephrol Dial Transplant (1995) 10: 637-642
© 1995 European Renal Association-European Dialysis and Transplant Association

research-article

Myoinositol incorporation into lymphocytes of chronic renal failure patients is impaired

P. Bartnicki1,, M. Luciak1, Z. Zbróg1 and H. Tchórzewski2

12nd Department of Internal Medicine WAM, Lódz, Poland 2Department of Pathophysiology and Immunology WAM, Lódz, Poland

Correspondence and offprint requests to: Correspondence and offprint requests to: Dr med. Piotr Bartnicki, 2nd Department of Internal Medicine, WAM, ul. Zeromskiego 113, 90-549 Lódz, Poland.

Incorporation of myo-[2-3H]-inosital into peripheral blood mononuclear cells (PBMNC) and T-cell enriched lymphocytes was evaluated in in-vitro experiments in chronic renal failure (CRF) patients and healthy subjects. Incorporation of myo-[2-3H]-inosital into the cells of CRF patients on conservative and haemodialysis treatment was found to be impaired in comparison with that observed in normal cells. Following PHA stimulation of the cells of CRF patients myo-[2-3H]-inosital incorporation decreased even further, while it increased in normal cells. Five hour haemodialysis session significantly depressed myoinositol incorporation into PBMNC, while its incorporation into T-cell enriched lymphocytes remained unaffected. Myoinositol incorporation into PBMNC and T-cell enriched lymphocytes was inhibited by prostaglandins and leukotrienes and was inversely related to the extent of pertussis toxinsensitive G protein activation. Reduced myoinositol incorporation into uraemic PHA-stimulated PBMNC may depend at least in part on their enhanced PGE2 and LTB4 release accompanied by increased intracellular cAMP production. In CRF impaired myoinositol incorporation into immune cells may prove the disarrangement in the early events of transmembrane signal transduction, which may share the responsibility for the cell-mediated immune defect in these patients.

Keywords: chronic renal failure; G proteins; leukotrienes; lymphocytes; myoinositol; prostaglandins


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