Nephrol Dial Transplant (2001) 16: 742-745
© 2001 European Renal Association-European Dialysis and Transplant Association
Effect of aluminium load on parathyroid hormone synthesis
1 Bone and Mineral Research Unit, Instituto Reina Sofía de Investigación and 2 Biochemistry Unit, Hospital Central de Asturias, Oviedo, Spain
Background. Aluminium overload leads to parathyroid hormone (PTH) suppression. However, it is unclear whether a decrease in synthesis or release of the hormone is mainly involved. The aim of this study was to assess the effect of an acute administration of aluminium on PTH synthesis and release in rats with chronic renal failure and secondary hyperparathyroidism.
Methods. The study was performed using 100 adult male Wistar rats (body weight 443±54 g). 7/8 nephrectomy was performed and the rats were maintained on a high dietary phosphorous intake. Five weeks after surgery, the rats were randomly divided into two groups, one loaded with aluminium (AlCl3) and the other given placebo. Aluminium or placebo were administered i.p. for two consecutive days. The placebo group received saline at the same pH as the aluminium solution. After 2 weeks, serum calcium, phosphorous, creatinine, PTH, and aluminium were measured. The parathyroid glands were removed and PTH messenger RNA (mRNA) was measured by northern blot. Intact PTH was measured by IRMA (Rat PTH®, Nichols Institute).
Results. No differences in serum PTH levels were found between the two groups after 5 weeks of renal failure. At the end of the study the rats given aluminium had higher aluminium levels than the placebo group and lower PTH levels. No significant differences were found for calcium, phosphorous, renal function, or body weight. PTH mRNA expression was lower in the aluminium group than in the placebo group.
Conclusion. The administration of aluminium in rats with chronic renal failure resulted in reductions in serum PTH and PTH mRNA. Thus far, previous studies had demonstrated that aluminium suppressed PTH release. The present findings suggest that PTH synthesis is also reduced.
Keywords: aluminium; chronic renal failure; hyper-parathyroidism; mRNA of pre-proPTH; PTH; rats
Correspondence and offprint requests to: Dr J. B. Cannata Andía, Bone and Mineral Research Unit, Hospital Central de Asturias, C/ Julián Clavería s/n. E-33006, Oviedo, Spain.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  G. M. London, S. J. Marchais, A. P. Guerin, P. Boutouyrie, F. Metivier, and M.-C. de Vernejoul Association of Bone Activity, Calcium Load, Aortic Stiffness, and Calcifications in ESRD J. Am. Soc. Nephrol., September 1, 2008; 19(9): 1827 - 1835. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. M. Brandenburg and J. Floege Adynamic bone disease--bone and beyond NDT Plus, June 1, 2008; 1(3): 135 - 147. [Full Text] [PDF]
|
|
|
|
|
|
G. M. London, C. Marty, S. J. Marchais, A. P. Guerin, F. Metivier, and M.-C. de Vernejoul Arterial Calcifications and Bone Histomorphometry in End-Stage Renal Disease J. Am. Soc. Nephrol., July 1, 2004; 15(7): 1943 - 1951. [Abstract] [Full Text] [PDF]
|
|