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Nephrol Dial Transplant (1989) 4: 611-617
© 1989 European Renal Association-European Dialysis and Transplant Association

research-article

Reactivity of Monoclonal Antibody P1 with Glomerular Basement Membrane in Thin-Membrane Nephropathy

F. E. Dische1,, I. P. Brooke1, S. J. Cashman2, A. Severn1, D. Taube1, V. Parsons1, M. Kershaw3, A. Reed3 and C. D. Pusey2

1Dulwich Hospital London 2Royal Postgraduate Medical School London 3Kingston Hospital Surrey, UK

Correspondence and offprint requests to: Correspondence and offprint requests to: Dr F. E. Dische, Department of Pathology, Dulwich Hospital, London SE22 8PT, UK

A monoclonal antibody, P1, possessing anti-glomerular basement membrane specificity similar to that of naturally-occurring Goodpasture antibody, was used to study renal biopsy material from patients with thin-membrane nephropathy. Immunofluorescence and immunoperoxidase techniques were employed to detect tissue localisation after sections had been incubated with P1. Staining of glomerular and tubular basement membranes in the 14 patients with thin-membrane nephropathy was similar to that in 10 subjects with various other renal diseases, whereas three patients with Alport's syndrome all gave diminished or absent staining, as has been reported previously. These observations confirm that Goodpasture antigen is present in the basement membranes in thin-membrane nephropathy and that it reacts normally with P1 antibody. They also add to the evidence that the lesions of thin-membrane nephropathy and Alport's syndrome are fundamentally different. The staining method may be used as a differential diagnostic test.

Keywords: Alport's syndrome; Anti-glomerular basement membrane antibody; Differential diagnosis; Hereditary nephropathy; Monoclonal antibody P1; Thin-membrane nephropathy


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