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Nephrol Dial Transplant (1992) 7: 865-870
© 1992 European Renal Association-European Dialysis and Transplant Association

research-article

Human cytomegalovirus in rejected kidney grafts; detection by polymerase chain reaction

J. Markovic-Lipkovski1, C. A. Müller2,, G. Engler-Blum1, F. Strutz1, W. Kühn1, T. Risler1, W. Lauchart3 and G. A. Müller1,

1Medical University Clinics, Department III Tubingen, Germany 2Section of Immunohematology and Transplantation Immunology Tubingen, Germany 3Surgery University Clinics, Eberhard-Karls University of Tubingen Tübingen, Germany

Correspondence and offprint requests to: Correspondence and offprint requests to: Dr G. A. Müller, Medical University Clinics, Department 3, Eberhard-Karls University of Tubingen 7400 Tubingen, Germany

Human cytomegalovirus (CMV) infections are frequently associated with graft rejection in the immunosuppressed patients following organ transplantation. Thirty-four tissue samples from rejected kidneys and 18 samples from normal adult kidneys obtained from autopsies were investigated for the presence of CMV-DNA by the polymerase chain reaction (PCR) and by immunohistochemistry. DNA extracted from renal tissues after proteinase K digestion was specifically amplified in 32 cycles using primers which flank a 147 bp DNA fragment of the immediate early CMV gene and analysed by slot-blot hybridization with digoxigenine-labelled detection oligonucleotides. CMV-DNA was detected by PCR in a range from 0.1 fg up to 100 fg in 14 (41%) rejected kidney transplants. Comparative immunohistological analysis revealed presence of CMV in only three biopsies of these rejected kidneys. Furthermore, CMV-DNA was also found in four of 18 (22%) normal donor kidneys. These results reveal that CMV is often present in rejected kidneys and that the infection can be transferred from the donor to the recipient, since the normal adult kidney appears to be a frequent site of latency for CMV.

No differences in local immunological changes, characterized by interstitial mononuclear leukocyte infiltration as well as by aberrant expression of HLAclass II antigens and of ICAM1 on proximal tubular epithelial cells, could be detected by further immu-nohistological analysis between grafted kidneys atlate stage of rejection with and without CMVinfection.

Keywords: human cytomegalovirus; kidney transplantation; polymerase chain reaction


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