Nephrol Dial Transplant (1994) 9: 389-394
© 1994 European Renal Association-European Dialysis and Transplant Association
research-article
Enhancement of reactive oxygen species production and cell surface markers expression due to haemodialysis
1Department of Nephrology Montpellier, France 2Department of Immunology, Hopital Lapeyronie Montpellier, France
Correspondence and offprint requests to: Correspondence and offprint requests to: B. Canaud, Department of Nephrology, Hopital Lapeyronnie, 34059 Montpellier, France
Leukocyte activation during haemodialysis (HD) was evaluated by reactive oxygen species (ROS) production and cell surface markers expression (CD1 lb: C3bi receptor and CD25: 1L2 receptor). Eight end-stage renal disease patients were exposed to three dialysis phases according to a A/B/A protocol study. During phase A polysuiphone (PS) membranes were used and during phase B cuprophane (CU) membranes were used. Each phase lasted 3 weeks. Timed samples were collected during the last session of each phase at 0, 15, and 30 mm of HD. Flow cytometry analysis was performed both on monocytes (MO), polymorphonuclears (PMN), and lymphocytes (Ly). Hydroethydine was used as a marker of cell-ROS production. Specific monoclonal antibodies were used to analyse the cell surface markers. CU increased ROS production in PMN and MO by 10- and 2.4-fold respectively and had no significant effect on Ly. CU enhanced 16-fold CD1Ib expression on PMN, and increased also CDllb and CD25 expression on MO by 7- and 40-fold respectively. On the contrary, PS did not affect either ROS production or cell surface markers expression in MO, PMN, Ly. We conclude that oxydative metabolism and cell surface markers expression of PMN and MO were significantly increased with cuprophane membranes and not with polysulphone membranes, suggesting that complex cell-cell interactions were involved in membrane-related bioincompatibility phenomena.
Keywords: haemodialysis; leukocyte activation; flow cytometr
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